Biolegend, 651354, Ultra-LEAF™ Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody, 1mg

PD-L1 is a type I transmembrane protein of 290 amino acids, and it is a member of the B7 family. Mouse PD-L1 has 70% amino acid identity to its human orthologue. Binding of PD-L1 to its receptor PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L1 induces IL-10 production in T cells stimulated with low levels of anti-CD3. PD-L1/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Disruption of the PD-L1 gene leads to up-regulated T cell responses and the generation of self-reactive T cells. Antibodies against PD-1 or PD-L1 leads to increased antitumor immunity. PDL1 has an important role in conferring fetomaternal tolerance in an allogeneic pregnancy model. Antibodies against PD-L1 lead to a breakdown in maternal tolerance to the fetus. PD-L1 shares its receptor with PD-L2 (CD273, B7-DC). PD-L2 has a more limited expression than PD-L1, being expressed on activated macrophages and dendritic cells. PD-L1 is expressed in many tumors and the interaction with its receptor activates signaling pathways that inhibit T-cell activity and therefore, the antitumor immune response. Antibodies targeting PD1 or PD-L1 block the PD1 pathway and reactivate T cell activity.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Mouse PD-1 (CD279)-Fc
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.04 µg/mL mouse PD-1 (CD279)-Fc (Cat. No. 764804) to immobilized 1 µg/mL recombinant mouse B7-H1 (PD-L1, CD274)-Fc (Cat. No. 758204). ND50 range: 0.02 - 0.1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3699078 (BioLegend Cat. No. 651353) AB_3699078 (BioLegend Cat. No. 651354)
Structure: Dimer
Distribution: APC, monocytes, dendritic cells, expressed in nonlymphoid tissues, and stromal cell.
Function: Enhances CD28-independent T-helper cell function, suppresses immune responses against autoantigens, and participates in fetomaternal tolerance.
Interaction: Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes
Ligand/Receptor: PD-1 (CD279)
Cell Type: B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area: Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References: Dong H, et al. 1999. Nat Med. 5:1365-9. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34. Latchman Y, et al. 2001. Nat Immunol. 2:261-8. Latchman YE, et al. 2004. Proc Natl Acad Sci USA. 101:10691. Guleria I, et al. 2005. J Exp Med. 202:231-7. Lin DY, et al. 2008. Proc Natl Acad Sci USA. 105:3011. Dai S, et al. 2014. Cell Immunol. 290:72-9. Melero I, et al. 2015. Nat Rev Cancer. 15:457-72.
Gene ID: 60533
UniProt: View information about CD274 on UniProt.org
Clone: W20151E
Regulatory Status: RUO
Other Names: CD274, Programmed cell death 1 ligand 1 (PD-L1), B7 homolog 1 (B7h1)
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.