Biolegend, 652419, Alexa Fluor® 700 anti-mouse Ki-67 Antibody, 25μg

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations: Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed Clark JT, et al. 2023. Cell Rep. 42:112147. PubMed Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed Zhang W, et al. 2022. Dev Cell. 57:329. PubMed Otano I, et al. 2021. Nat Commun. 12:7296. PubMed Hill DL, et al. 2021. Elife. 10:. PubMed Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed Xia L, et al. 2021. Cell Chemical Biology. 28(5):610-624.e5. PubMed Song W, et al. 2022. Immunity. 55:290. PubMed Jacobs L, et al. 2022. Cancer Gene Ther. 29:984. PubMed
RRID: AB_2564284 (BioLegend Cat. No. 652419) AB_2564285 (BioLegend Cat. No. 652420)
Structure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution: Nucleus and chromosome
Function: Required for cell cycle progression and proliferation
Interaction: Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143. 2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. 3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. 4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. 5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. 6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 17345
UniProt: View information about Ki-67 on UniProt.org
Clone: 16A8
Regulatory Status: RUO
Other Names: KIA, proliferation-related Ki-67 antigen
Isotype: Rat IgG2a, κ