Biolegend, 652505, Purified anti-His Tag Antibody, 1mg

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).
1mg
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated His-tag peptide
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml (Dilution 1:1000 - 1:5000). For immunocytochemistry, a concentration range of 0.1 - 0.5 μg/ml (Dilution 1:1000 - 1:5000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations: Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed Goosens VJ, et al. 2021. ACS Synth Biol. 10:3422. PubMed Lam B, et al. 2021. Cell Rep. 37:109838. PubMed Shigemori S, et al. 2017. J Dairy Sci. 10.3168/jds.2017-12872. PubMed Mamedov T, et al. 2017. PLoS One.. 10.1371/journal.pone.0183589. PubMed Namai F, et al. 2017. Curr Microbiol. 10.1007/s00284-017-1354-3. PubMed Vunnam N, et al. 2020. Biochemistry. 59:3856. PubMed Zoulias N, et al. 2021. Curr Biol. S0960-9822:01407. PubMed Williford JM, et al. 2019. Sci Adv. 5:eaay1357. PubMed Liu L, et al. 2019. J Cell Biol. 218:644. PubMed Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed Manrique-Suárez V, et al. 2021. Proteins. 89:1508. PubMed Yumoto K, et al. 2020. Antioxidants (Basel). 9:00. PubMed Keshwani M, et al. 2015. Biochem J. 472: 329 - 338. PubMed
RRID: AB_11204080 (BioLegend Cat. No. 652501) AB_11204427 (BioLegend Cat. No. 652502) AB_2564271 (BioLegend Cat. No. 652505)
Function: Recombinant poly-histidine tag detection
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about His Tag on UniProt.org
Clone: J099B12
Regulatory Status: RUO
Other Names: Histidine Tag
Isotype: Mouse IgG1, κ