Biolegend, 652602, Purified anti-IRF5 Antibody, 100μg

IRF-5 is a transcription factor belonging to the interferon regulatory factor family. It controls induction of type I IFN genes as well as inflammatory cytokines and chemokines through TLRs and the MyD88-TRAF6 pathway. Several genetic variations of IRF-5 have been reported as a risk factor of Systemic lupus erythematosus (SLE), which is caused by excess IFNα induction.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human IRF-5 recombinant protein (176-240 aa).
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2-10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Internal testing suggests this clone is not suitable for intracellular flow cytometry.
Product Citations: Breuer J, et al. 2014. Ann Neurol. 739:75. PubMed
RRID: AB_2561469 (BioLegend Cat. No. 652601) AB_2561470 (BioLegend Cat. No. 652602)
Structure: 498 aa with predicted molecular weight of 56 kD. The protein contains a DNA binding domain (IRF tryptophan pentad repeat) and a poly-Glutamate region.
Distribution: Shuttles between the nucleus and the cytoplasm.
Function: The transcription factor IRF-5 participates in the virus-mediated activation of interferon and regulates gene expression induced by cell growth, differentiation, and apoptosis.
Interaction: IKKα phosphorylates IRF-5 and induces formation of IRF-5 dimers. TRAF6 mediates K63-linked ubiquitination of IRF-5, required for nuclear translocation of IRF-5 and regulation of target genes. IRF-5 assemblies with CBP/p300 to initiate transcription.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References: 1. Balkhi MY, et al. 2010. Cell. Signal. 1:117. 2. Balkhi MY, et al. 2008. Mol. Cell. Biol. 28:7296. 3. Salloum R and Niewold TB. 2011. Transl. Res. 157:326. 4. Barnes BJ, et al. 2003. J. Biol. Chem. 278:16630. 5. Rullo OJ, et al. 2010. Ann. Rheum. Dis. 69:611.
Gene ID: 3663
UniProt: View information about IRF5 on UniProt.org
Clone: 11F4A09
Regulatory Status: RUO
Other Names: Interferon regulatory factor 5 (IRF-5)
Isotype: Mouse IgG2b, κ