Biolegend, 652802, Purified anti-AIM2 Antibody, 100μg

AIM2 is one of the human IFN-inducible proteins, encoding a 39 kD protein. It contains a 200 amino acid repeat, which is a signature of the HIN-200 family (hematopoietic IFN-inducible nuclear proteins). Expression of AIM2 can inhibit cell growth and tumor formation, possibly through inducing apoptosis and suppressing nuclear factor-κ B transcriptional activity and desensitizing tumor necrosis factor-alpha-mediated nuclear factor-κ B activation. Furthermore, the gene for AIM2 shows a high frequency of mutations that are associated with microsatellite unstable colorectal cancers. AIM2 plays a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. AIM2 is also essential for mediating inflammatory reactions triggered by cytoplasmic DNA. AIM2 is an important sensor for different pathogens through the binding of cytoplasmic double-stranded DNA through HIN-200 domain and formation of inflammatsomes including ASC/AIM2. This complex then triggers caspase-1 activation. Recent studies also show that AIM2/ASC complex acts as a novel caspase-8 activation platform.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment, corresponding to amino acids 1-186 of human AIM2 with a GST tag.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Reported by the developer, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5-2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence staining3 and immunoprecipitation2.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Application References(PubMed link indicates BioLegend citation): Pierini R, et al. 2012. Cell Death Differ. 19:1709. (WB) Fernandez-Alnemri T, et al. 2009. Nature 7237:509. (IP) Sagulenko V, et al. 2013. Cell Death Differ. 9:1149. (IF)
Product Citations: Su S et al. 2018. Cell. 175(2):442-457 . PubMed
RRID: AB_2561539 (BioLegend Cat. No. 652801) AB_2561540 (BioLegend Cat. No. 652802)
Structure: 343 amino acids with predicted molecular weight of 39 kD.
Distribution: Cytosol.
Function: Tumor suppressor which may act by repressing NF-κ B transcriptional activity.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Antigen References: 1. DeYoung KL, et al. 1997. Oncogene 15:453. 2. Choubey D, et al. 2000. FEBS Lett. 474:38. 3. Chen IF, et al. 2006. Mol. Cancer Ther. 5:1. 4. Roberts TL, et al. 2009. Science 323:1057. 5. Jones JW, et al. 2010. Proc. Natl. Acad. Sci. USA 107:9771. 6. Fernandes-Alnemri T, et al. 2010. Nat. Immunol. 11:385. 7. Schroder K and Tschopp J. 2010. Cell 140:821. 8. Jin T, et al. 2012. Immunity 36:561.
Gene ID: 9447
UniProt: View information about AIM2 on UniProt.org
Clone: 3B10
Regulatory Status: RUO
Other Names: Absent in melanoma 2 (AIM2), PYHIN4, interferon-inducible protein AIM2
Isotype: Mouse IgG1, κ