Biolegend, 653206, Alexa Fluor® 647 anti-mouse Aiolos Antibody, 100μg

Aiolos, an Ikaros family member, is also known as Izkf3. It has a molecular weight of 58 kD and shows 70% homology to the Ikaros isoform, Ik-1, particularly in the DNA binding and C-terminal domains. Aiolos can form a heteromer with Ikaros or exist as a homodimer. The homomeric complex is a potent transcriptional activator.  Unlike Ikaros, Aiolos is expressed in late fetal thymus and adult lymphoid organs. Among the adult hematopoietic progenitors, Aiolos is expressed at low levels in mulitpotent but not in pluripotent stem cells. When these multipotent progenitors become more restricted to T and B lymphoid pathways there is an upregulation in Aiolos expression. The highest expression of Aiolos is detected in the spleen (especially in B-cells) followed by thymus and bone marrow, and cannot be detected in the non-lymphoid organs. Aiolos plays an important role in lymphocyte proliferation and differentiation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal half of mouse Aiolos fused to GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, immunoprecipitation1, and Western blotting2.Does not cross-react with human.View more applications data for a different format of this clone in our Scientific Poster Library.This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.
Application References(PubMed link indicates BioLegend citation): Laszlo G, et al. 1993. J. Immunol. 150:5252. (ICFC, IP) Thompson EC, et al. 2007. Immunity 26:335. (WB)
Product Citations: Tauber M, et al. 2023. J Exp Med. :220. PubMed
RRID: AB_2563238 (BioLegend Cat. No. 653205) AB_2563239 (BioLegend Cat. No. 653206)
Structure: 509 amino acids with predicted MW of 58 kD
Distribution: Nucleus, cytoplasm Restricted to lymphoid lineage, expressed in B, NK and T cells
Function: Transcription factor that plays an important role in the regulation of lymphocyte differentiation; plays an essential role in regulation of B-cell differentiation, proliferation, and maturation to an effector state
Interaction: Homodimer and heterodimer with other IKAROS family members; interacts with IKZF4, IKZF5, IKZF1, HRAS, FOXP3, and BCL21L isoform Bcl-X(L); associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A
Cell Type: B cells, NK cells, T cells
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Morgan B, et al. 1997. EMBO J. 16:2004. 2. Wang JH, et al. 1998. Immunity 9:543. 3. Cariappa A, et al. 2001. Immunity 14:603.
Gene ID: 22780
UniProt: View information about Aiolos on UniProt.org
Clone: 8B2
Regulatory Status: RUO
Other Names: IKAROS family zinc finger 3 (Ikzf3), zinc finger protein Aiolos, Zfpn1a3, Znfn1a3
Isotype: Mouse IgG1, κ