Biolegend, 653702, Purified anti-Oct4 (Oct3) Antibody, 100μg

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human Oct4 recombinant protein (1-141aa).
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration of 2.5 µg/ml is recommended. For ChIP applications, the suggested use of this reagent is 1:50 to 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Claassen JN et al. 2019. Stem Cell Res. 1723:12:00 . PubMed Ingham V, et al. 2014. J Neuroinflammation. 115:11. PubMed Raad S, et al. 2022. Stem Cell Res. 60:102711. PubMed Guo Y et al. 2018. Stem cell research. 29:139-142 . PubMed Zhu T, et al. 2021. Stem Cell Research. 55:102502. PubMed Fu Q, et al. 2021. Stem Cell Research. 54:102428. PubMed Wu S, et al. 2021. Stem Cell Research. 53:102330. PubMed Grzela DP, et al. 2020. Stem Cell Research. 72.65138889. PubMed Xu Z, et al. 2021. Stem Cell Research. 53:102359. PubMed McLenachan S et al. 2019. Stem Cell Res. 1723:17:00 . PubMed
RRID: AB_2561766 (BioLegend Cat. No. 653701) AB_2561767 (BioLegend Cat. No. 653702)
Structure: Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
Distribution: Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
Interaction: SOX2, SOX8, SOX10, androgen receptor, and ETS2
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Antigen References: 1. Niwa H, et al. 2000. Nature Genet. 24:372. 2. Wernig M, et al. 2007. Nature 448:318. 3. Pesce M, et al. 2001. Stem Cells 19:271.
Gene ID: 5460
UniProt: View information about Oct4 on UniProt.org
Clone: 3A2A20
Regulatory Status: RUO
Other Names: POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
Isotype: Mouse IgG2b, κ