Biolegend, 653706, Alexa Fluor® 488 anti-Oct4 (Oct3) Antibody, 100tests

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human Oct4 recombinant protein (1-141aa).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Product Citations: Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed Wang W, et al. 2021. Front Pharmacol. 12:707940. PubMed Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 47:101906. PubMed Ababneh NA, et al. 2021. Stem Cell Research. 54:102358. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 48:101925. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 47:101891. PubMed Petters J, et al. 2020. Stem Cell Research. 72.42222222. PubMed Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed V ölkner C, et al. 2020. Stem Cell Research. 41:101606.. PubMed Guzzardo P, et al. 2017. Sci Rep.. 10.1038/s41598-017-16931-z. PubMed
RRID: AB_2562250 (BioLegend Cat. No. 653705) AB_2562251 (BioLegend Cat. No. 653706)
Structure: Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
Distribution: Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
Interaction: SOX2, SOX8, SOX10, androgen receptor, and ETS2
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Antigen References: 1. Niwa H, et al. 2000. Nature Genet. 24:372. 2. Wernig M, et al. 2007. Nature 448:318. 3. Pesce M, et al. 2001. Stem Cells 19:271.
Gene ID: 5460
UniProt: View information about Oct4 on UniProt.org
Clone: 3A2A20
Regulatory Status: RUO
Other Names: POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
Isotype: Mouse IgG2b, κ