Biolegend, 654002, Purified anti-IDO1 Antibody, 100μg

IDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant mouse IDO1.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Protein G affinity purified.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 2E2/IDO1 reacts with mouse IDO1. It does not appear to react with mouse IDO2. Clone 2E2/IDO1 is the same clone as anti-IDO1 clone 2E2.6.
Product Citations: Ji R, et al. 2021. PLoS One. 16:e0258204. PubMed Yuk H, et al. 2021. Antioxidants (Basel). 10:. PubMed Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
RRID: AB_2561779 (BioLegend Cat. No. 654001) AB_2561780 (BioLegend Cat. No. 654002)
Structure: 403 amino acids with a predicted molecular weight of 45 kD.
Distribution: Cytoplasm
Function: Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.
Interaction: This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.
Biology Area: Cell Biology, Immunology
Antigen References: 1. Habara-Ohkubo A, et al. 1991. Gene 105:221. 2. Munn DH, et al. 2002. Science 297:1867. 3. Frumento G, et al. 2002. J. Exp. Med. 196:459. 4. Muller AJ, et al. 2005. Nature Med. 11:312. 5. Lob S, et al. 2009. Nature Rev. Cancer 9:445.
Gene ID: 15930
UniProt: View information about IDO1 on UniProt.org
Clone: 2E2/IDO1
Regulatory Status: RUO
Other Names: Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase
Isotype: Mouse IgG1, κ