Biolegend, 654652, Purified anti-DNER Antibody, 100μg

DNER is a type I transmembrane protein primarily expressed in the nervous system. First identified as a non-canonical Notch ligand, DNER plays a crucial role in neuronal differentiation. DNER is particularly involved in neuron-glial interactions, and abnormalities in DNER function have been associated with neurological disorders and impaired brain development. DNER is expressed in many tissue types and plays a role in a variety of diseases such as COPD, periodontitis, and breast cancer.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein corresponding to the N terminus of human DNER
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). This product is not recommended for use in Western blot (WB), flow cytometry (FC), intracellular flow cytometry (ICFC) or immunocytochemistry (ICC).
RRID: AB_3699080 (BioLegend Cat. No. 654651) AB_3699080 (BioLegend Cat. No. 654652)
Structure: DNER is a 737 amino acid protein with an expected molecular weight of 78.5 kD.
Distribution: Cell membrane
Function: Notch activator
Interaction: α- and β- spectrin, GFAP
Ligand/Receptor: Notch 1, Notch 2
Cell Type: Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References: Eiraku M, et al. 2005. Nat Neurosci. 8:873-80. Saito SY and Takeshima H 2006. Cerebellum. 5:227-31. Hiseh FY, et al. 2013. Dev Biol. 375:1-12.
Gene ID: 92737
UniProt: View information about DNER on UniProt.org
Clone: W23166E
Regulatory Status: RUO
Other Names: Delta and Notch-like epidermal growth factor-related receptor (DNER), BET, UNQ26/PRO299, HE60
Isotype: Rat IgG2b, κ