Biolegend, 654803, PE anti-BATF Antibody, 25tests

BATF is a basic leucine zipper transcription factor belonging to the ATF superfamily. BATF forms heterodimers with Jun proteins, which in turn binds to AP-1 consensus sites. The BATF/Jun heterodimers have significantly reduced transcriptional activity compared to Fos/Jun heterodimers. Therefore, induction of BATF expression suppresses AP-1 target genes by competing for Fos/Jun binding sequences. BATF is expressed at low levels in hematopoietic cells and can be induced in response to a variety of stimuli. BATF knockout mice show defects in cytokine expression and in the differentiation of T helper cell subsets and B cells, but not in the proliferation of lymphocytes. These findings suggest that BATF is a tissue-specific regulator for AP-1 activity and plays an essential role in the regulation of immune responses. During early EBV infection, BATF expression is induced by EBNA2 proteins, subsequently preventing infected B cells from entering apoptotic and lytic pathways. This indicates that BATF functions as a modulator to maintain latency status of virus-infected cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human BATF recombinant protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
RRID: AB_2563516 (BioLegend Cat. No. 654803) AB_2563517 (BioLegend Cat. No. 654804)
Structure: 14 kD protein containing a bZIP (basic-leucine zipper) domain, which mediates dimerization with Jun family members.
Distribution: Nucleus. Expressed most abundantly in hematopoietic tissues.
Function: AP-1 transcription complexes regulate gene expression in response to various stimuli, such as cytokines, growth factors, stress, and infection. BATF belongs to the AP-1/ATF superfamily and negatively regulates AP-1 mediated transcription when it forms heterodimers with Jun proteins.
Interaction: Forms heterodimers with c-Jun, JunB, and JunD. Interacts with IFI35, IRF4, and IRF8 through bZIP domain.
Cell Type: B cells, Dendritic cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Betz BC, et al. 2010. J. Exp. Med. 207:933. 2. Dorsey MJ, et al. 1995. Oncogene 11:2255. 3. Echlin DR, et al. 2000. Oncogene 19:1752. 4. Johansen LM, et al. 2003. J. Virol. 77:6029. 5. Schraml BU, et al. 2009. Nature 460:405. 6. Tussiwand R, et al. 2012. Nature 490:502.
Gene ID: 10538
UniProt: View information about BATF on UniProt.org
Clone: 9B5A13
Regulatory Status: RUO
Other Names: B-cell-activating transcription factor, Basic leucine zipper transcriptional factor ATF-like, SFA-2, BATF1
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.