Biolegend, 655204, Alexa Fluor® 647 anti-TCF1 (TCF7) Antibody, 100tests

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial TCF1 recombinant protein (116-334 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Product Citations: McMurray JL, et al. 2022. Cell Rep. 39:110858. PubMed Wu B, et al. 2023. J Immunother Cancer. 11: . PubMed Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed Sung BY, et al. 2022. J Clin Invest. 132:. PubMed Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
RRID: AB_2566619 (BioLegend Cat. No. 655203) AB_2566620 (BioLegend Cat. No. 655204)
Structure: 384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
Distribution: Nucleus
Function: TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Interaction: TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers, TCRs
Antigen References: 1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207. 2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060. 3. Wang R, et al. 2011. J. Immunol. 187:5964. 4. Weber BN, et al. 2011. Nature 476:63. 5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777. 6. Staal FJ, et al. 1999. Int. Immunol. 11:317.
Gene ID: 6932
UniProt: View information about TCF1 on UniProt.org
Clone: 7F11A10
Regulatory Status: RUO
Other Names: T-cell-specific transcription factor 1, transcription factor 7, TCF7
Isotype: Mouse IgG1, κ