Biolegend, 655552, Purified anti-ITPR1 Antibody, 100μg

ITPR1 is primarily involved in calcium signaling within cells. Located on the membrane of the endoplasmic reticulum (ER), it functions as a calcium channel. Upon binding to inositol 1,4,5-trisphosphate (IP3), a second messenger molecule, ITPR1 facilitates the release of calcium ions from the ER into the cytoplasm. This calcium release plays a crucial role in regulating various cellular processes, including muscle contraction, neurotransmitter release, and gene expression. Mutations in the ITPR1 gene are associated with neurological disorders such as spinocerebellar ataxia and Gillespie syndrome.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein located near the C terminus of human ITPR1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). This product (W22192B) is not recommended for use in Western Blot (WB) due to the presence of a non-specific band in addition to the expected band. This product is not recommended for use in intracellular flow cytometry (ICFC) or immunocytochemistry (ICC).
RRID: AB_3699081 (BioLegend Cat. No. 655551) AB_3699081 (BioLegend Cat. No. 655552)
Structure: ITPR1 is a 2758 amino acid protein with a predicted molecular weight of 313.9 kD.
Distribution: Endoplasmic reticulum
Function: IP3-gated calcium channel
Interaction: Calmodulin, FKBP1A, Ankyrin-B, HOMER proteins
Ligand/Receptor: Inositol 1,4,5-trisphosphate (IP3)
Biology Area: Signal Transduction, Synaptic Biology
Molecular Family: Endoplasmic Reticulum Markers
Antigen References: Tolonen JP, et al. 2024. Mov Disord. 39:141-151. Putney JW, 1987. Am J Physiol. 252:G149-57. van de Leemput J, et al. 2007. PLoS Genet. 3:e108.
Gene ID: 3708
UniProt: View information about ITPR1 on UniProt.org
Clone: W22192B
Regulatory Status: RUO
Other Names: Inositol 1,4,5-trisphosphate-gated calcium channel (ITPR1), IP3 receptor isoform 1 (IP3R 1, InsP3R1), Inositol 1,4,5, trisphosphate receptor type 1
Isotype: Rat IgG2a, κ