Biolegend, 655702, Purified anti-IRF3 Antibody, 100μg

IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial IRF-3 recombinant protein (200-427 a.a.)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Affinity purified
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Ta A, et al. 2023. Nat Commun. 14:2035. PubMed He X, et al. 2017. PLoS One. 13(6):e1006347. PubMed Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed
RRID: AB_2562201 (BioLegend Cat. No. 655701) AB_2562202 (BioLegend Cat. No. 655702)
Structure: Human IRF-3 has 427 amino acids with predicted Mw 47.2kD and observed Mw 52kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9kD and observed Mw 45kD.
Distribution: Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.
Function: IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.
Interaction: CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Lin R, et al. 1999. Mol. Cell Biol. 19:959. 2. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325. 3. Molle C, et al. 2010. J. Immunol. 184:1784.
Gene ID: 3661
UniProt: View information about IRF3 on UniProt.org
Clone: 12A4A35
Regulatory Status: RUO
Other Names: Interferon regulatory factor-3 (IRF-3)
Isotype: Mouse IgG2b, κ