Biolegend, 655802, Purified anti-c-REL Antibody, 100μg

c-Rel is a member of the Rel/NF-κB transcription factor family that forms homo or heterodimers with family members such as RelA/p65, RelB, NF-κB1/p105, and NF-κB2/p100. The NF-κB complex is retained in cytoplasm by the inhibitor IκB protein in unstimulated cells. Upon activation, IκB is degraded via the ubiquitin-proteasome pathway, which results in nuclear translocation of the active NF-κB complex. While other NF-κB transcription factors are expressed in most cell types, relatively higher levels of c-REL expression are found in hematopoietic cells. c-REL is thought to play key roles in regulating proliferation, differentiation, and survival of B and T lymphocytes.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial c-REL recombinant protein (453-587 a.a.)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Affinity purified
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2562203 (BioLegend Cat. No. 655802)
Structure: 619 amino acids, predicted molecular weight of 69 kD, contains a RHD (Rel-like) domain responsible for dimerization and DNA binding, contains two C-terminal transactivation domains
Distribution: Cytoplasm, translocated to the nucleus upon activation
Function: Transcription factor belonging to the NF-κB family, regulates various biological processes including tumorgenesis, inflamation, immune response, cell proliferation, and differentiation
Interaction: Forms homo or heterodimer with other NF-κB family members; interacts with NKIRAS1, NFKBIB, NFKBIE, IRF-8, and Foxp3
Cell Type: B cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers, TCRs
Antigen References: 1. Bash J, et al. 1997. Mol. Cell Biol. 17:6526. 2. Chen G, et al. 2010. J. Immunol. 185:2350. 3. Gilmore TD, et al. 2011. Genes Cancer 2:695. 4. Ferch U, et al. 2007. Nat. Immunol. 8:984. 5. Sanjabi S, et al. 2005. Genes Dev. 19:2138. 6. Yamazaki T, et al. 2003. Nat. Immunol. 4:780.
Gene ID: 5966
UniProt: View information about c-REL on UniProt.org
Clone: 2F5A22
Regulatory Status: RUO
Other Names: REL, V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog, Proto-Oncogene C-Rel
Isotype: Mouse IgG2b, κ