Biolegend, 656011, APC anti-IRF7 Antibody, 25tests

IRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial IRF7 recombinant protein (127-503 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2936700 (BioLegend Cat. No. 656011) AB_2936700 (BioLegend Cat. No. 656012)
Structure: 503 amino acids, predicted molecular weight of 54 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding
Distribution: Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus
Function: Transcription factor that transactivates proinflamatory cytokines and type I interferon genes in response to virus infection
Interaction: Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References: 1. Owens BM, et al. 2012. PLoS One. 7:e41050. 2. Yu Y, et al. 2010. Immunity. 33:863. 3. Liang Q, et al. 2011. J. Immunol. 186:1001. 4. Buss C, et al. 2010. J. Immunol. 184:3072. 5. Joo CH, et al. 2007. J. Virol. 81:8282. 6. Balachandran S, et al. 2007. J. Immunol. 178:2429.
Gene ID: 3665
UniProt: View information about IRF7 on UniProt.org
Clone: 12G9A36
Regulatory Status: RUO
Other Names: Interferon regulatory factor-7 (IRF-7)
Isotype: Mouse IgG2b, κ