Biolegend, 656110, Alexa Fluor® 488 anti-SOX2 Antibody, 100μg

SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human SOX recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Ceholski D, et al. 2017. Stem Cell Res. 10.1016/j.scr.2017.06.015. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Chen G, et al. 2020. Stem Cell Research. 72.58402778. PubMed Alexandrova S, et al. 2016. Development. 143: 24 - 34. PubMed Coccini T, et al. 2022. Curr Protoc. 2:e423. PubMed
RRID: AB_2563956 (BioLegend Cat. No. 656109) AB_2563957 (BioLegend Cat. No. 656110)
Structure: 317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution: Nucleus
Function: Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction: Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References: 1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228. 2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725. 3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772. 4. Jeong CH, et al. 2010. Stem Cells 28:2141. 5. Xiang R, et al. 2011. Br. J. Cancer 104:1410. 6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.
Gene ID: 6657
UniProt: View information about SOX2 on UniProt.org
Clone: 14A6A34
Regulatory Status: RUO
Other Names: SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
Isotype: Mouse IgG1, κ