Biolegend, 656114, Brilliant Violet 421™ anti-SOX2 Antibody, 100μg

SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human SOX recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Yamasaki S, et al. 2022. iScience. 25:103657. PubMed Matsuda M, et al. 2020. Nature. 124:580. PubMed Shiba T, et al. 2019. J Allergy Clin Immunol. 144:1438. PubMed
RRID: AB_2566262 (BioLegend Cat. No. 656114)
Structure: 317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution: Nucleus
Function: Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction: Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References: 1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228. 2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725. 3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772. 4. Jeong CH, et al. 2010. Stem Cells 28:2141. 5. Xiang R, et al. 2011. Br. J. Cancer 104:1410. 6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.
Gene ID: 6657
UniProt: View information about SOX2 on UniProt.org
Clone: 14A6A34
Regulatory Status: RUO
Other Names: SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.