Biolegend, 656251, Purified anti-p53 Antibody, 25μg

The p53 protein plays a crucial role as a tumor suppressor by regulating the cell cycle, promoting DNA repair, and inducing apoptosis in response to cellular stressors such as DNA damage. Its function is tightly regulated by proteins like Mdm2 and Mdm4, which inhibit p53 activity under normal conditions and prevent unnecessary cell death. In clinical research, mouse models have been invaluable for understanding p53 role in cancer biology. These models help elucidate how p53 mutations contribute to tumor development and progression, providing insights that are directly translatable to human cancers. The study of p53 in mice has also facilitated the development of targeted therapies aimed at reactivating p53 function in tumors, highlighting its importance in advancing cancer treatment.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of mouse p53 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize human p53.
RRID: AB_3717124 (BioLegend Cat. No. 656251) AB_3717124 (BioLegend Cat. No. 656252)
Structure: Mouse p53 consists of 390 amino acids and its molecular weight is approximately 53 kDa.
Distribution: Mainly nuclear localization. Can be found in cytoplasm.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family: Phospho-Proteins, Tumor Suppressors
Antigen References: Wang H, et al. 2023. Sig Transduct Target Ther. 8:92. Sabapathy K, et al. 2019. J Mol Cell Biol. 11:317-329. Loughery J, et al. 2014. Nucleic Acids Research. 42:7666-80. Marei HE, et al. 2021. Cancer Cell International. 21:703.
Gene ID: 22059
UniProt: View information about p53 on UniProt.org
Clone: W22179B
Regulatory Status: RUO
Other Names: P53, Tumor protein 53, LFS1, Tumor suppressor p53, Cellular tumor antigen P53, Antigen NY-CO-13, Phosphoprotein p53
Isotype: Rat IgG2b, λ