Biolegend, 656701, Purified anti-CCL11 (Eotaxin) Antibody, 500μg

CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. In response to allergens, CCL11, which participates in a variety of allergic diseases, promotes accumulation of eosinophils. CCL11 production is upregulated in response to several proinflammatory cytokines, such as IL-4, IL-13, TNF-α, and IFN-α, produced by mast cells and Th2 lymphocytes. CCL11 has been reported to stimulate eosinophil release from bone marrow, resulting in an elevated level of circulating eosinophils. In addition, CCL11 facilitates proliferation and metastasis of tumor cells expressing the CCL11 receptor, CCR3. CCL11 has been shown to induce an angiogenic response by CCR3-positive microvascular endothelial cells.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CCL11 recombinant protein (Gly24-Pro97) expressed in E. coli
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2562411 (BioLegend Cat. No. 656701)
Distribution: Secreted; Expressed in epithelial cells, smooth muscle cells, dendritic cells, and fibroblasts
Function: CCL11 selectively recruits eosinophils through binding to the chemokine receptor CCR3 during allergic response.
Interaction: Eosinophils
Ligand/Receptor: CCR3
Cell Type: Epithelial cells, Dendritic cells, Fibroblasts, Eosinophils
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Conroy DM, et al. 2001. Respir. Res. 2:150. 2. Levina V, et al. 2009. Clin. Cancer Res. 15:2647. 3. Salcedo R, et al. 2001. J. Immunol. 166:7571. 4. Kaehler J, et al. 2006. J. Investig. Med. 54:446. 5. Hadjicharalambous C, et al. 2004. J. Allergy Clin. Immunol. 113:657. 6. Crump MP, et al. 1998. J. Biol. Chem. 273:22471.
Gene ID: 6356
UniProt: View information about CCL11 on UniProt.org
Clone: 16D10A40
Regulatory Status: RUO
Other Names: Eotaxin-1, small inducible cytokine subfamily A, member 11 (SCYA11)
Isotype: Rat IgG2a, κ