Biolegend, 656802, Purified anti-Nestin Antibody, 100μg

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Fusion protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunohistochemistry use on formalin-fixed paraffin-embedded tissue (IHC-P), antigen retrieval with Citrate Buffer, 10X (Cat. No. 420902) is recommended.
Application References(PubMed link indicates BioLegend citation): Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
Product Citations: Salgado B, et al. 2023. Microorganisms. 11:. PubMed Di Stefano B, et al. 2019. Cell Stem Cell. 25:622. PubMed Hu YB, et al. 2021. Aging Cell. 20:e13454. PubMed Feng H, et al. 2021. Stem Cell Research. 53:102346. PubMed Zelenova EA, et al. 2021. Cells. 10:. PubMed
RRID: AB_2562473 (BioLegend Cat. No. 656801) AB_2562474 (BioLegend Cat. No. 656802)
Structure: 1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD.
Distribution: Cytosol
Function: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction: Vimentin, alpha-internexin and FHOD3
Cell Type: Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Intermediate Filaments
Antigen References: Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59. Krupkova O Jr, et al. 2010. Neoplasma. 57:291. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.
Gene ID: 10763
UniProt: View information about Nestin on UniProt.org
Clone: 10C2
Regulatory Status: RUO
Other Names: NES
Isotype: Mouse IgG1, κ