Biolegend, 657409, Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3) Antibody, 100microl

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
100microl
Verified Reactivity: Mouse, Rat, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Fusion protein
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Zeng H, et al. 2024. Acta Neuropathol Commun. 23:12. PubMed
RRID: AB_2566704 (BioLegend Cat. No. 657409)
Structure: 450 amino acids with predicted molecular weight of 50 kD
Distribution: Cytosol
Function: Plays important roles in neuronal cell proliferation and differentiation
Interaction: Alpha tubulin, kinesin and dynein
Cell Type: Mature Neurons
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Antigen References: 1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851. 2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015. 3. Locher H, et al. 2013. Differentiation. 85:173. 4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461. 5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726. 6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.
Gene ID: 10381
UniProt: View information about Tubulin beta-3 on UniProt.org
Clone: AA10
Regulatory Status: RUO
Other Names: β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
Isotype: Mouse IgG2a, κ