Biolegend, 657602, Purified anti-IRF1 Antibody, 100μg

IRF1 is the first identified member of the interferon regulatory transcription factor family. Its expression is induced in response to pathogen infection or the stimulation of several cytokines, including type I and II IFN, IL-1, IL-6, and TNF-α. Upon stimulation, IRF1 is transcriptionally activated by STAT1 and NFκB transcription factors via the GAS (interferon-gamma-activated sequence) element in the IRF1 promoter. IRF1 plays important  roles in mediating immune response during infection and development of T cells and NK cells. In addition to the immunomodulatory functions, IRF1 has also shown to be involved in the regulation of cell cycle progression, tumor suppression, and apoptosis. IRF1-mediated transcription can be repressed by IRF2 through competing for the same binding region on the promoters.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human IRF1 recombinant protein (147-325 a.a.)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Affinity purified.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted at 4°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Velásquez LN, et al. 2017. J Leukoc Biol. 101:759. PubMed Bailey KA, et al. 2019. FASEB J. 33:12888. PubMed
RRID: AB_2562631 (BioLegend Cat. No. 657602)
Structure: 325 amino acids, predicted Mw is 37 kD and observed MW is 45kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding.
Distribution: Nucleus.
Function: IRF1 is a transcription factor, regulating expression of IFN and IFN-inducible genes. It is also involved in regulating cell proliferation, apoptosis, tumor suppression, and various immune responses.
Interaction: Forms homodimers. Interacts with p300, MyD88, and PIAS3.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Transcription Factors
Antigen References: 1. Yang W, et al. 2012. PLoS One 7:e50932. 2. Schwartz JL, et al. 2011. Int. J. Breast Cancer. 2011:912102. 3. Horiuchi M, et al. 2011. J. Neuroinflammation. 8:8. 4. Frontini M, et al. 2009. Nucleic Acids Res. 37:1073. 5. Yarilina A, et al. 2008. Nat. Immunol. 9:378. 6. Kano S, et al. 2008. Nat. Immunol. 9:34.
Gene ID: 3659
UniProt: View information about IRF1 on UniProt.org
Clone: 13H3A44
Regulatory Status: RUO
Other Names: Interferon regulatory factor 1 (IRF-1)
Isotype: Mouse IgG2a, κ