Biolegend, 658010, PE anti-SPI1 (PU.1) Antibody, 100tests

SPI1 is a transcription factor belonging to the E26-transformation-specific (Ets) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as occurring malignancy. Overexpression of SPI1 blocks erythroid differentiation and inhibits cell death.  Mice carrying a mutant SPI1 allele show decreased SPI1 expression and develop acute myeloid leukaemia (AML), suggesting the role of SPI1 in oncogenesis.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human SPI1 recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Product Citations: Nott A, et al. 2021. Nature Protocols. 16(3):1629-1646. PubMed
RRID: AB_2616862 (BioLegend Cat. No. 658009) AB_2616863 (BioLegend Cat. No. 658010)
Structure: 270 amino acids, predicted molecular weight of 31 kD; contains a C-termianl ETS domain responsible for DNA binding.
Distribution: Nucleus.
Function: SPI1 is a transcription factor required for the development of lymphoid and myeloid cells.
Interaction: SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Cell Type: B cells, Dendritic cells, Neutrophils
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hikami K, et al. 2011. Arthritis Rheum. 63:755. 2. Zakrzewska A, et al. 2010. Blood 116:e1. 3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391. 4. Pospisil V, et al. 2011. EMBO J. 30:4450. 5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922. 6. Rimmelé P, et al. 2010. Cancer Res. 70:6757.
Gene ID: 6688
UniProt: View information about SPI1 on UniProt.org
Clone: 7C6B05
Regulatory Status: RUO
Other Names: Spleen focus forming virus (SFFV) proviral integration oncogene, Transcription factor PU.1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.