Biolegend, 658102, Purified anti-FOXO1 Antibody, 100μg

FOXO1 is a transcription factor, belonging to the O subfamily of forkhead box-containing proteins. The forkhead proteins are substrates of Akt/PKB and glucocorticoid-induced kinases that mediate insulin signaling. FOXO1 binds to the conserved insulin-responsive elements and acts as a transcriptional activator. The FOXO1 target genes are involved in cell metabolism, growth, and differentiation. Upon insulin stimulation, FOXO1 proteins are phosphorylated by AKT/PKB through the PI3K pathway, resulting in the translocation of FOXO1 proteins from the nucleus to the cytoplasm. The expression of FOXO1 target genes are then inhibited by FOXO1. FOXO1 has been reported to regulate maturation and class-switch recombination of B cells, as well as participating in regulatory T cell function. FOXO1-deficient mice are embryonic lethal, suggesting that FOXO1 has crucial functions in development which cannot be compensated by other FoxO family members.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human FOXO1 recombinant protein (544-655aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 20 μg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 8.0 - 10. µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2562793 (BioLegend Cat. No. 658102)
Structure: 655 amino acids, predicted molecular weight of 70 kD. Contains a C-terminal trans-activating domain and a Fork-head domain responsible for DNA binding.
Distribution: Cytoplasm, Nucleus
Function: FOXO1 is a transcription factor mediating Insulin signaling. It regulates expression of the genes involved in cell growth, differentiation, and metabolism.
Interaction: FOXO1 interacts with RUNX2, NLK, SIRT1, PRMT1, SKP2, ATF4, and p300.
Cell Type: B cells, Tregs
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Trinh DL, et al. 2013. Blood. 121:3666. 2. Ouyang W, et al. 2012. Nature. 491:554. 3. Ferdous A, et al. 2012. Transcription. 3:221. 4. Cifarelli V, et al. 2012. Mol. Endocrinol. 26:1213. 5. Kamagate A, et al. 2008. Cell Cycle. 7:3162. 6. Kousteni S. 2012. Bone. 50:437.
Gene ID: 2308
UniProt: View information about FOXO1 on UniProt.org
Clone: 2F8B08
Regulatory Status: RUO
Other Names: Forkhead box protein O1A (FOXO1A), Forkhead in rhabdomyosarcoma (FKHR)
Isotype: Mouse IgG1, κ