Biolegend, 658202, Purified anti-PAX9 Antibody, 100μg

PAX9 is a transcription factor belonging to the PAX family, which is composed of nine members defined by the presence of a paired box domain responsible for DNA binding. PAX9 is expressed at the E10 stage and downregulated at the bell stage. It is controlled by Fgf/Bmp signaling and plays an essential role in tooth development. PAX9 deficient mice fail to form teeth and show developmental arrest at the bud stage. In humans, heterozygous mutation in PAX9 is associate with tooth agenesis. These findings support the role of PAX9 in tooth development.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human PAX9 recombinant protein (131-341 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody binds non-specifically to a protein over 250kDa therefore it is not recommended to use in immunofluorescence staining.
Product Citations: Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
RRID: AB_2562877 (BioLegend Cat. No. 658202)
Structure: 341 amino acids in human and 342 amino acids in mouse, with predicted molecular weight of 36 kD. Contains a paired box domain responsible for DNA binding.
Distribution: Nucleus.
Function: PAX9 is a transcription factor essential for tooth development.
Interaction: PAX9 interacts with KDM5B.
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Almeida CV, et al. 2010. J. Appl. Oral Sci. 18:482. 2. Wang Y, et al. 2009. Cells Tissues Organs. 189:80. 3. Pereira TV, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:5676. 4. Mensah JK, et al. 2004. J. Biol. Chem. 279:5924. 5. Peters H, et al. 1998. Genes Dev. 12:2735. 6. Guala A, et al. 2008. Int. J. Paediatr. Dent. 18:441.
Gene ID: 5083
UniProt: View information about PAX9 on UniProt.org
Clone: 14D10A24
Regulatory Status: RUO
Other Names: Paired box protein Pax-9
Isotype: Mouse IgG2a, κ