Biolegend, 658302, Purified anti-SPHK1 Antibody, 100μg

SPHK1 is a lipid kinase that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P) which is a key sphingolipid signaling molecule involved in cell growth, survival, differentiation, and motility. As an important regulator of ceramide, sphingosine, and S1P, SPHK1 plays important roles in immunological, inflammatory diseases, and cancer. SPHK1 is overexpressed in various types of cancers, and upregulation of SPHK1 has been associated with tumor angiogenesis and resistance to radiation and chemotherapy. Agents targeting the SPHK1 have the potential as therapeutics for cancer, immunological, and inflammatory disorders.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SPHK1 peptide-KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2562878 (BioLegend Cat. No. 658302)
Structure: 384-470 amino acids, predicted molecular weight of 45-60 kD.
Distribution: Cytoplasm and nucleus.
Function: SPHK1 is a lipid kinase that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate( S1P). It plays important roles in immunological, inflammatory diseases, and cancer.
Interaction: ACY1, calmodulin, and SPHKAP.
Ligand/Receptor: Sphingosine.
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Wallington-Beddoe CT, et al. 2013. Br. J. Haematol. 161:623. 2. Hisano Y, et al. 2012. J. Biochem. 152:305. 3. Carr JM, et al. 2013. Rev. Med. Virol. 23:73. 4. Maceyka M, et al. 2012. Trends Cell Biol. 22:50. 5. Shida D, et al. 2008. Curr. Drug Targets. 9:662.
Gene ID: 8877
UniProt: View information about SPHK1 on UniProt.org
Clone: 1A5SC
Regulatory Status: RUO
Other Names: Sphingosine Kinase 1, SPK1, SK1
Isotype: Mouse IgG1, κ