Biolegend, 658502, Anti-c-Myc Tag (9E10) Affinity Gel, 500microl

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.
500microl
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino acids 408-439, C-terminal region of human c-myc
Formulation: 50% anti-c-Myc-tag (9E10) antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3.
Preparation: Affinity purified.
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C. The unopened product is stable for one year upon arrival.
Application: Purification of c-Myc tagged fusion protein from bacteria lysates - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Resin binding capacity is greater than 0.5 mg/mL.
Application Notes: *Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
Application References(PubMed link indicates BioLegend citation): Rao H, et al. 2001. Nature. 410:955. Peters CS, et al. 2001. J Biol Chem. 276:13718. Munro S, Pelham HR. 1987. Cell. 48:899. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations: Liu L, et al. 2023. Cancers (Basel). 15:. PubMed Liu L, et al. 2022. Sci Adv. 8:eadd8928. PubMed Zhang G, et al. 2016. J Virol. 90: 2306 - 2315. PubMed Shamay-Ramot A, et al. 2015. PLoS Genet. 11: 1005702. PubMed
RRID: AB_3106269 (BioLegend Cat. No. 658502)
Cell Targets: N-terminal, C-terminal tagged and internal fusion protein
Biology Area: Cell Biology, Protein Purification, Protein Synthesis
Antigen References: Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217. Battey J, et al. 1983. Cell 34:779. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID: 4609
UniProt: View information about c-Myc on UniProt.org
Clone: 9E10
Regulatory Status: RUO
Other Names: myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Isotype: Mouse IgG1, κ