Biolegend, 658802, Purified anti-XBP-1s Antibody, 100μg

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of the human HLA-DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 is upregulated as part of the ER stress response, also known as the unfolded protein response.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial mouse XBP-1s recombinant protein (162-267aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For ChIP application, use 2 µg of antibody and 10 µg of chromatin per IP. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone 9D11A43 recognizes spliced XBP-1 (XBP-1s/isoform 2), which is 371 amino acids and migrates around 55 kD on the SDS-PAGE.
Additional Product Notes: Using this antibody at a ratio higher than 1:5 (µg antibody per ug of chromatin) will increase background
Product Citations: Bezu L, et al. 2018. Cell Death Differ. 25:1375. PubMed Liu Z, et al. 2023. J Neuroinflammation. 20:93. PubMed Sundaram A et al. 2017. eLife. 6 pii: e27187. PubMed Yu W, et al. 2022. Acta Pharm Sin B. 12:2315. PubMed Saito A et al. 2017. Journal of neurochemistry. 144(1):35-49 . PubMed Dion W, et al. 2022. Sci Adv. 8:eabl4150. PubMed Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed Sicari D, et al. 2020. FEBS J. 287:27. PubMed
RRID: AB_2562960 (BioLegend Cat. No. 658802)
Distribution: Nucleus.
Function: Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: MHC Antigens, Nuclear Markers
Antigen References: 1. Liou HC, et al. 1990. Science 247:1581. 2. Ponath PD, et al. 1993. J. Biol. Chem. 268:17074. 3. Takahashi S, et al. 2002. Prostate 50:154.
Gene ID: 7494
UniProt: View information about XBP-1s on UniProt.org
Clone: 9D11A43
Regulatory Status: RUO
Other Names: XBP-1 isoform2, Tax-responsive element-binding protein 5 (TREB5), XBP2
Isotype: Mouse IgG2a, κ