Biolegend, 659201, Purified anti-mTOR Antibody, 25μg

mTOR (mammalian target of rapamycin) is a 289 kD member of the PI3 kinase family, containing HEAT, FRB, and FATC domains. mTOR is expressed in the nucleus and cytoplasm, and is a target for cell-cycle arrest and the immunosuppressive effects of FKBP12-rapamycin complex. mTOR controls ribosome biogenesis and cell growth, and acts as an ATP sensor. mTOR is repressed by nutrient deprivation and mitochondrial uncoupling. mTOR is the target of PI3K-regulated protein kinase, AKT/PKB (phosphorylated at Thr 2446 and Ser2448). mTOR has also been shown to bind RAPTOR, which negatively regulates mTOR kinase activity, and is also inhibited by tuberin-hamartin tumor suppressor complex. The binding of RAPTOR to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro , and it strongly enhances the mTOR kinase activity toward p70-alpha.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human mTOR recombinant protein (1186-1385 aa) expressed in E. coli.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Sohrabi Y, et al. 2019. Front Immunol. 9:3155. PubMed Yang Y, et al. 2022. Nat Commun. 13:5469. PubMed
RRID: AB_2563155 (BioLegend Cat. No. 659201) AB_2563156 (BioLegend Cat. No. 659202)
Structure: 2549 amino acids encode a protein with predicted MW of 289 kD.
Distribution: Nucleus, Cytoplasm
Function: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.
Interaction: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40, and DEPTOR; also part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1, and DEPTOR.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Antigen References: 1. Brown EJ, et al. 1994. Nature. 369:756. 2. Dennis P, et al. 2001. Science. 294:1102. 3. Fingar D, et al. 2002. Genes Dev. 16:1472. 4. Nojima H, et al. 2003. J. Biol. Chem. 278:15461.
Gene ID: 2475
UniProt: View information about mTOR on UniProt.org
Clone: 6H9B10
Regulatory Status: RUO
Other Names: FKBP12-rapamycin complex-associated protein 1, FK506 binding protein 12-rapamycin associated protein 1, FRAP1
Isotype: Mouse IgG1, κ