Biolegend, 659702, Purified anti-NLRC4 (IPAF) Antibody, 100μg

NLRC4 , also known as IPAF, is a member of the NOD-like receptor (NLR) family. The NLR proteins share a conserved DNA-binding NACHT domain, a caspase recruitment domain (CARD), and a leucine-rich repeat (LRR) domain responsible for pathogen pattern recognition. NLRC4 was found to play an important role in the innate immune response against bacterial infection. During Gram-negative bacteria infections, such as Shigella, Salmonella, Legionella, or Pseudomonas, NLRC4 detects and is activated by translocated bacterial flagellin or PrgJ-like rod proteins subsequently resulting in the assembly of the NLRC4 inflammasome. The activated inflammasome drives proteolytic activation of caspase-1, leading to the processing and secretion of the proinflamatory cytokines IL-1β and IL-18.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human NLRC4 recombinant protein (1-88 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Baker GJ, et al. 2016. Oncoimmunology. e1163461:5. PubMed Fytianos K, et al. 2017. ACS Nano. 375:11. PubMed
RRID: AB_2563347 (BioLegend Cat. No. 659702)
Structure: 1024 amino acids, predicted molecular weight of 116 kD. Contains a CARD domain, a NACHT domain, and 14 leucine-rich repeats.
Distribution: Cytoplasm.
Function: NLRC4 is involved in the assembly of the inflammasome complex, activation of pro-caspase-1, and induction of matured proinflammatory cytokines during bacterial infections.
Interaction: Foms homo-oligomer in NLRC4 inflammasome. Interacts with Caspase-1, ASC, NOD2, NALP1, and BCL10.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Cai S, et al. 2012. J. Immunol. 188:5623. 2. Franchi L, et al. 2012. Nat. Immunol. 13:449. 3. Carvalho FA, et al. 2012. Mucosal Immunol. 5:288. 4. Tomalka J, et al. 2011. PLoS Pathog. 7:e1002379. 5. Pereira MS, et al. 2011. J. Immunol. 187:6447. 6. Sauer JD, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:12419.
Gene ID: 58484
UniProt: View information about NLRC4 on UniProt.org
Clone: 6H9B13
Regulatory Status: RUO
Other Names: NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein (CLAN), Caspase recruitment domain-containing protein 12 (CARD12), Ice protease-activating factor (IPAF)
Isotype: Mouse IgG2a, κ