Biolegend, 660052, Purified anti-Acetyl-CoA Carboxylase alpha Phospho (Ser79) Antibody, 100μg

Acetyl-CoA carboxylase alpha (ACCα or ACACA) is a crucial enzyme in fatty acid biosynthesis. This enzyme catalyzes the conversion of acetyl-CoA to malonyl-CoA, a key step in the production of fatty acids. This enzyme plays a vital role in various cellular functions, including energy storage, membrane formation, and hormone production. The activity of Acetyl-CoA carboxylase alpha is tightly regulated by factors such as insulin, glucagon, and dietary fat intake, ensuring a balance between fatty acid synthesis and catalysis. Phosphorylation at serine 79 (Ser79) is a critical regulatory mechanism in response to changes in energy status and nutrient availability. This specific phosphorylation event inhibits the enzyme activity, thereby reducing the production of malonyl-CoA, a key precursor in fatty acid synthesis. Dysregulation of Acetyl-CoA carboxylase alpha is associated with several metabolic disorders, including obesity, type 2 diabetes, non-alcoholic fatty liver disease (NAFLD), and certain cancers. Hence, targeting Acetyl-CoA carboxylase alpha can help reduce lipid accumulation and alleviate mitochondrial dysfunction, making it a potential therapeutic target for these conditions.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic human Acetyl-CoA Carboxylase peptide phosphorylated at Serine 79
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody on immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with either Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704) or Citrate Buffer (Cat. No. 420901).
RRID: AB_3683492 (BioLegend Cat. No. 660051) AB_3683492 (BioLegend Cat. No. 660052)
Structure: This enzyme is a 2346 amino acid protein with a molecular weight of approximately 265 kDa. It can exist as a monomer, homodimer, and homotetramer.
Distribution: The Acetyl-CoA carboxylase alpha is located in the cytoplasm.
Function: Function: - Fatty Acid Biosynthesis: Catalyzes the conversion of acetyl-CoA to malonyl-CoA, a crucial step in fatty acid synthesis - Energy Storage: Plays a role in storing energy by facilitating the production of fatty acids, which are later stored as triglycerides - Membrane Formation: Contributes to the synthesis of fatty acids that are essential components of cell membranes - Hormone Production: Involved in the biosynthesis of fatty acids that serve as precursors for various hormones Regulation: -Phosphorylation: - Inhibition: Phosphorylation at serine 79 (Ser79) inhibits ACCα activity, reducing fatty acid synthesis - Activation: Dephosphorylation activates ACCα, promoting fatty acid synthesis Hormonal Control: - Insulin: Activates ACCα, enhancing fatty acid synthesis - Glucagon and Epinephrine: Inhibit ACCα, reducing fatty acid synthesis Nutrient Availability: - High Carbohydrate Diet: Increases ACCα activity - High Fat Diet: Decreases ACCα activity
Interaction: BRCA1: In its inactive phosphorylated form, ACCα interacts with the BRCT domains of BRCA1, which prevents ACCα dephosphorylation and inhibits lipid synthesis. MID1IP1: This interaction promotes the oligomerization of ACCα, increasing its enzymatic activity. AMPK (Adenosine Monophosphate-Activated Protein Kinase): Phosphorylates ACCα, leading to its inactivation and subsequent inhibition of lipid synthesis Actin Filaments: ACCα can associate with actin filaments, which may help regulate its activity and localization.
Antigen References: Dong J, et al. 2024. Journal of Translational Medicine. 22:196. Yang W, et al. 2024. BCM Med. 22:376. Xie J, et al. 2024. Front Biosci. 29:143. Jokinen MJ, et al. 2024. Trends Pharmacol Sci. 45:319.
Gene ID: 31
UniProt: View information about Acetyl-CoA Carboxylase 1 on UniProt.org
Clone: A23002B
Regulatory Status: RUO
Other Names: ACACA, ACCα, ACC-Alpha, ACC1, Acetyl-Coenzyme A Carboxylase alpha, ACC
Isotype: Mouse IgG2b, κ