Biolegend, 661451, Purified anti-HOXB4 Antibody, 25μg

HOXB4 is a member of the Homeobox (HOX) gene family, which plays a role in regulating embryonic development and maintaining adult tissue homeostasis. HOXB4 is a sequence-specific transcription factor with a homeobox DNA-binding domain. In hematopoiesis, HOXB4 enhances the self-renewal capacity of hematopoietic stem cells (HSCs), which are responsible for the continuous production of blood cells. Overexpression of HOXB4 in HSCs has been shown to lead to an expansion of these stem cells without inducing leukemogenesis. By binding to specific DNA sequences, HOXB4 activates a network of target genes that promote stem cell proliferation and inhibit differentiation, thus maintaining the stem cell pool. HOXB4 also plays a pivotal role in organogenesis, particularly in the development of the spinal cord and the patterning of the vertebral column. During embryonic development, HOXB4 is expressed in a specific spatial and temporal manner, contributing to the formation of structures along the anterior-posterior axis. Its expression helps determine the identity and positional information of various segments, ensuring that body parts develop in the correct locations. In vertebrates, this function is crucial for the proper segmentation and alignment of the spine, as well as for the formation of the nervous system. Furthermore, aberrations in HOXB4 expression or function have been associated with various pathological conditions, including cancers. Dysregulated HOXB4 expression can lead to the uncontrolled proliferation of cells, contributing to tumorigenesis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human HOXB4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is not suitable for use in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P) or immunocytochemistry (ICC). This antibody does not cross react with mouse HOXB4 in Western blotting (WB).
RRID: AB_3675188 (BioLegend Cat. No. 661451) AB_3675188 (BioLegend Cat. No. 661452)
Structure: Contains a homeobox DNA-binding domain
Distribution: Expressed in embryonic tissue and hematopoietic stem cells/localized in nucleus
Function: A transcription factor that regulates hematopoietic stem cell self-renewal or development of anterior-posterior axis
Interaction: Binds to homeobox DNA sequence
Biology Area: Neuroscience, Stem Cells, Transcription Factors
Antigen References: Gould A, et al. 1998. Neuron. 21:39-51. Antonchuk J, et al. 2002. Cell. 109:39-45. Kyba M, et al. 2002. Cell. 109:29-37. Bowles KM, et al. 2006. Stem Cells. 24:1359-69.
Gene ID: 3214
UniProt: View information about HOXB4 on UniProt.org
Clone: W22219B
Regulatory Status: RUO
Other Names: Homeobox protein Hox-B4, Homeobox protein Hox-2.6, Homeobox protein Hox-2F, Homeobox B4, HOX2F
Isotype: Rat IgG2a, κ