Biolegend, 662102, Purified anti-C/EBPα Antibody, 100μg

The CCAAT-enhancer binding proteins (C/EBP) are members of the basic region/leucine zipper (bZIP) transcription factors, that bind to the consensus CCAAT sequence in DNA. C/EBPα is expressed in two isoforms: p42 and p30 by ultilizing different translational start codons. The C/EBPα p30 isoform has little to no transactivating activity and acts as an inhibitor. C/EBPα regulates expression of various myeloid-specific genes and is required for myeloid differentiation. C/EBPα has been found to play a key role in adipogenesis, since inhibition of C/EBPα blocks adipocyte differentiation. C/EBPα also regulates cell cycle progression through interactions with cyclin-dependent kinases (CDK) and by inducing the expression of CDK inhibitor p21. Mutations in the C/EBPα gene are associated with the development of acute myeloid leukemia.
100μg
Verified Reactivity: Human
Reported Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide (AKQLALAGLFPYQPPPP) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product weakly reacts with Mouse.
Product Citations: Oliveros-Matus P, et al. 2020. Front Pharmacol. 11:303. PubMed Li Y, et al. 2018. Front Immunol. 32:415. PubMed
RRID: AB_2564224 (BioLegend Cat. No. 662102)
Structure: 358 amino acids with a predicted molecular weight of 38 kD; contains a bZIP domain responsible for DNA binding.
Distribution: Nucleus
Function: C/EBPα is a bZIP transcription factor that regulates proliferation and differentiation of myeloid cells and adipocytes.
Interaction: C/EBPα forms heterodimers with C/EBPβ and γ; interacts with CDK2, CDK4, PRDM16, UBN1, and ZNF638.
Cell Type: Neutrophils
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. De Obaldia ME, et al. 2013. Nat. Immunol. 14:1277. 2. Di Tullio A, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:17016. 3. Fan HY, et al. 2011. Mol. Endocrinol. 25:253. 4. Cai DH, et al. 2008. Oncogene. 27:2772. 5. Khanna-Gupta A. 2008. Blood Cells Mol. Dis. 41:77. 6. Janardhan HP. 2008. Sci. Signal. 1:jc2.
Gene ID: 1050
UniProt: View information about C/EBPalpha on UniProt.org
Clone: 16C12B70
Regulatory Status: RUO
Other Names: CCAAT/enhancer-binding protein alpha, C/EBP-A
Isotype: Mouse IgG2a, κ