Biolegend, 662402, Purified anti-IκB-α Antibody, 100μg

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.
100μg
Verified Reactivity: Human, Mouse, Rat, Hamster
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human IκB-α recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/ml (1:1000 - 1:5000 dilution). For immunocytochemistry, the suggested use is 0.5 - 1.0 µg/ml (1:500-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.
Product Citations: Buono L, et al. 2023. J Clin Med. 12:. PubMed Smaldone G, et al. 2021. Sci Rep. 11:18237. PubMed Millen S, et al. 2020. Retrovirology. 17:30. PubMed
RRID: AB_2564258 (BioLegend Cat. No. 662402)
Structure: Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution: Cytoplasm
Function: Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction: NFkB1, SUMO4, p53, ribosomal S6 kinase 1
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Tumor Suppressors
Antigen References: 1. Baeuerle PA. 1998. Cell 95:729. 2. Hoffmann A, et al. 2002. Science 298:1241. 3. Ito CY, et al. 1995. Genomics 29:490.
Gene ID: 4792
UniProt: View information about IkappaB-alpha on UniProt.org
Clone: 3D6C02
Regulatory Status: RUO
Other Names: Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA
Isotype: Mouse IgG2b, κ