Biolegend, 662409, Brilliant Violet 421™ anti-IκB-α Antibody, 25μg

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.
25μg
Verified Reactivity: Human, Mouse, Rat, Hamster
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human IκB-α recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250-1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.
RRID: AB_2728495 (BioLegend Cat. No. 662409)
Structure: Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution: Cytoplasm
Function: Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction: NFkB1, SUMO4, p53, ribosomal S6 kinase 1
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Tumor Suppressors
Antigen References: 1. Baeuerle PA. 1998. Cell 95:729. 2. Hoffmann A, et al. 2002. Science 298:1241. 3. Ito CY, et al. 1995. Genomics 29:490.
Gene ID: 4792
UniProt: View information about IkappaB-alpha on UniProt.org
Clone: 3D6C02
Regulatory Status: RUO
Other Names: Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA
Isotype: Mouse IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.