Biolegend, 663104, Purified anti-, 100μg

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.
100μg
Verified Reactivity: E. coli
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-1.0 ug/mL (1:500-1:5000). It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Lesley SA and Burgess RR. 1989. Biochemistry 28:7728.
Product Citations: Chang S, et al. 2022. Nat Struct Mol Biol. 29:932. PubMed Gibson JA, et al. 2022. Proc Natl Acad Sci U S A. 119:e2210115119. PubMed Hummels KR, et al. 2023. Nature. 615:300. PubMed Sanderlin AG, et al. 2022. J Bacteriol. 204:e0018222. PubMed Prentice JA, et al. 2022. J Bacteriol. 204:e0024922. PubMed Liang Q, et al. 2022. Front Cell Infect Microbiol. 12:1081614. PubMed Park SY, et al. 2022. Cell. 185:513. PubMed Di Venanzio G, et al. 2019. Nat Commun. 10:2763. PubMed Hernandez–Doria JD, et al. 2018. Cell Host Microbe. 23:607. PubMed Shin JH, et al. 2021. EMBO Rep. 22:e51790. PubMed Tomasek D, et al. 2020. Nature. 583:473. PubMed Han K, et al. 2021. MBio. 12:. PubMed Shin JH, et al. 2020. Biochem Biophys Res Commun. 528:493. PubMed Guo MS et al. 2018. Cell. 175(2):583-597 . PubMed Martin NR, et al. 2021. Nat Microbiol. 6:910. PubMed Weaver AI, et al. 2022. Elife. 11:. PubMed Zingl FG, et al. 2020. Cell Host & Microbe. 27(2):225-237.e8.. PubMed Bridges AA, et al. 2021. Elife. 10:. PubMed Yang N, et al. 2020. Mol Microbiol. 114:423. PubMed Guzzo M, et al. 2021. Dev Cell. 56:2145. PubMed
RRID: AB_2687386 (BioLegend Cat. No. 663104)
Structure: α is a 36 kD subunit of the E. coli RNA polymerase.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 947794
UniProt: View information about RNA Polymerase alpha on UniProt.org
Clone: 4RA2
Regulatory Status: RUO
Other Names: RNA polymerase subunit alpha, Transcriptase subunit alpha, pez, phs, sez
Isotype: Mouse IgG1