Biolegend, 663703, Anti-, 100microl

Sigma transcription factor controlling the regulation of genes is required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon, and other genes by modifying the affinity of RNA polymerase for the promoters.
100microl
Verified Reactivity: E. coli
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Preparation: Dried ascites
Storage & Handling: Store lyophilized antibody at -20°C.
Application: WB - Quality tested ELISA - Verified FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconstitution. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Previously item# W0009 from Neoclone. Clone 1RS1 does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthobacter, or Borrelia. It recognizes epitope 33-256. Additional reported applications (for the relevant formats) include: functional assay.
Additional Product Notes: Non-specific band may be observed at 60 kD.
Application References(PubMed link indicates BioLegend citation): Nguyen LH, et al. 1993. Biochemistry 32:11112.
Product Citations: Bray AS, et al. 2022. MBio. 13:e0359521. PubMed Kurylo CM, et al. 2018. Cell Rep. 25:236. PubMed Xu L et al. 2017. Structure (London, England : 1993). 25(12):1795-1808 . PubMed
RRID: AB_2564522 (BioLegend Cat. No. 663703)
Structure: Sigma S (σS) is a 38 kD transcription factor of E. coli RNA polymerase.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 947210
UniProt: View information about RNA Sigma S on UniProt.org
Clone: 1RS1
Regulatory Status: RUO
Other Names: Sigma 38, rpoS, appR, katF, nur, otsX, sigS, RNA Polymerase
Previously: NeoClone Catalog# W0009
Isotype: Mouse IgG1