Biolegend, 664906, Purified anti-RNA Polymerase II RPB1 Antibody, 100μg

RPB1 is the catalytic and largest component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with RPB2, the second largest subunit. Polymerase II (Pol II) is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relatively to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft, and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single DNA template strand of the promoter is positioned within the central active site cleft of Pol II. Then, a bridging helix emanates from RPB1 and crosses the cleft near the catalytic site, which acts as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations during each neuocleotide addition. This promotes translocation of Pol II. Pol II moves on the template during transcription elongation. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II's largest subunit (RPB1), which serves as a platform for assembling factors that regulate transcription initiation, elongation, termination, and mRNA processing. It can act as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, being able to conform as both a replicate and transcriptase for the viral RNA circular genome.
100μg
Verified Reactivity: Human, Mouse
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Wheat germ RNA Polymerase II
Formulation: Phosphate-buffered solution, pH 7.2.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested ICC, ChIP - Verified FA, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. The suggested dilution for ChIP application is 1:300 - 1:500 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 8WG16 recognizes YSPTSPS in the C-terminal domain. The antibody cross-reacts with wheat, yeast, mouse, c. elegans, x. laevis, and most eukaryotic RNAPII. Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation, functional assay, and immunoprecipatation.
Application References(PubMed link indicates BioLegend citation): Edwards AM, et al. 1990. Proc. Natl. Acad. Sci. USA. 87:2122. Thompson NE, et al. 1990. J. Biol. Chem. 265:7069. Thompson NE, et al. 1989. J. Biol. Chem. 264:11511.
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RRID: AB_2565554 (BioLegend Cat. No. 664906)
Structure: RPB1 is a 220 kD subunit of RNA polymerase II and has 1970 amino acids.
Distribution: Nucleus; ubiquitous expressed in all cells
Function: Core element of RNA polymerase II transcription machinery.
Interaction: COMPASS (COMplex of Proteins ASsociated with Set1), SET2, BRE1
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Chávez S, et al. 2016. CUrr. Genet. 62:701.
Gene ID: 5430200202540292
UniProt: View information about RNA Polymerase II RPB1 on UniProt.org
Clone: 8WG16
Regulatory Status: RUO
Other Names: RNA polymerase II sub-unit 1, RPB1, CTD repeat YSPTSPS, C- terminal domain repeat YSPTSPS, DNA-directed RNA polymerase II subunit A, RNA-directed RNA polymerase II subunit RPB1, POLR2A, POLR2
Isotype: Mouse IgG2a