Biolegend, 665004, Purified anti-RNA Polymerase II RPB3 Antibody, 100μg

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The RNA polymermase II subunit RPB3 is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft and is known to be involved in transcription termination.
100μg
Verified Reactivity: Yeast
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: 0.22 µm filtered protein solution is in PBS, pH 7.2.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Application: WB - Quality tested ELISA - Verified ChIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3. Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
Product Citations: Yurko N, et al. 2017. Mol Cell. 68:913. PubMed Maudlin IE, et al. 2021. RNA Biol. 18:782. PubMed Neurohr GE, et al. 2019. Cell. 176:1083. PubMed Jeronimo C, et al. 2019. Cell Rep. 28:1206. PubMed Jeronimo C, et al. 2021. Molecular Cell. 81:3542. PubMed Ryu HY, et al. 2019. EMBO J. 38:e102003. PubMed Collin P, et al. 2019. Mol Cell. 73:655. PubMed Abraham K, et al. 2016. Nucleic Acids Res. 44: 8870 - 8884. PubMed
RRID: AB_2565221 (BioLegend Cat. No. 665004)
Structure: RPB3 is a 45 kD subunit of the yeast RNA polymerase II.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 854791
UniProt: View information about RNA Polymerase II RPB3 on UniProt.org
Clone: 1Y26
Regulatory Status: RUO
Other Names: RNA polymerase II sub-unit 3, RPB3, POLR2C
Isotype: Mouse IgG1