Biolegend, 665205, Purified anti-RNA Polymerase II RPB5 Antibody, 25μg

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The common components of RNA polymerases I, II, and III synthesize ribosomal RNA precursors, mRNA precursors, and many functional non-coding RNAs and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB5 is part of the lower jaw that surrounds the central large cleft and is believed to grab the incoming DNA template. It seems to be the major component in this process.
25μg
Verified Reactivity: Yeast
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Previously item# W0014 from Neoclone.
RRID: AB_2876742 (BioLegend Cat. No. 665205) AB_2876742 (BioLegend Cat. No. 665206)
Structure: RPB5 is a 27 kD subunit of yeast RNA polymerase II.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 852451
UniProt: View information about RNA Polymerase II RPB5 on UniProt.org
Clone: 3Y11
Regulatory Status: RUO
Other Names: DNA-directed RNA polymerases I, II, and III subunit RPABC1, PolR2E, RNA polymerases I, II, and III subunit ABC1, RPA7, RPC9
Isotype: Mouse IgG1, κ