Biolegend, 666052, Purified anti-, 100μg

Most prokaryotes such as E. coli express a single core RNA polymerase to transcribe RNA from DNA. RNA Sigma 70, also known as rpoD, is the major housekeeping member of the sigma factor subunit family which are required for core RNA polymerase to bind to promoters and initiate transcription. RNA sigma 70 is also required for the unwinding of DNA strands to allow access to RNA polymerase.
100μg
Verified Reactivity: E. coli
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This recombinant antibody is derived from the original clone 2G10. We sequenced the complementarity-determining regions (CDRs) from the 2G10 hybridoma and grafted them into a mouse IgG1, κ Fc framework. Clone 2G10 specifically recognizes the major sigma factor across multiple bacterial species but shows no reactivity against Borrelia burgdorferi. Clone 2G10 is known to target epitope 470-486 in the major sigma factor of E. coli.
RRID: AB_3699084 (BioLegend Cat. No. 666051) AB_3699084 (BioLegend Cat. No. 666052)
Structure: RNA Sigma 70 is a 70 kD transcription factor of E. coli RNA polymerase.
Distribution: Bacterial cytoplasm
Interaction: Core RNA polymerase subunits (RpoA, RpoB, RpoC, RpoZ)
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Gruber T, et al. 2003. Annual review of microbiology. 441-66. PMID: 14527287
Gene ID: 947567
UniProt: View information about Sigma 70 on UniProt.org
Clone: 2G10.RecM1
Regulatory Status: RUO
Other Names: RNA polymerase sigma factor RpoD, sigma 70, sigma D, σ70, P00579
Isotype: Mouse IgG1, κ