Biolegend, 666801, Purified anti-MLKL Antibody, 25μg

MLKL has a four-helix bundle (4HB) domain at the N-terminal and a C-terminal PsKD connected via a two-helix linker. MLKL is important in the necroptotic signaling pathways. During the process, phosphorylated RIP3 (receptor-interacting protein 3) is recruited and phosphorylates MLKL which can induce the oligomerization of MLKL. Oligomerized MLKL translates to the plasma membrane, destroying the membrane integrity and inducing necroptosis. Knockdown or inhibition of MLKL prohibits necrosis. High expression of MLKL and RIP3 are reported in inflammatory bowel disease. MLKL is also involved in endosomal trafficking and extracellular vesical generation, which is necroptosis independent.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human recombinant MLKL Protein (1-193 a.a.) expressed in E. Coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.5 µg per ml (1:200 - 1:2000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2801124 (BioLegend Cat. No. 666801) AB_2801125 (BioLegend Cat. No. 666802)
Distribution: Cytoplasm
Function: Necroptosis
Interaction: RIP3
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Gene ID: 197259
UniProt: View information about MLKL on UniProt.org
Clone: W17104A
Regulatory Status: RUO
Other Names: MLKL, HMLKL
Isotype: Rat IgG2a, κ