Biolegend, 667001, Purified anti-PIK3R2 Antibody, 25μg

Phosphoinositide-3-kinase (PI3 kinase) is a lipid kinase that plays a critical role in cell signaling through generation of phosphoinositides at the plasma membrane in response to growth factor and hormone stimulation. Activation of PI3K is essential for cell growth, migration, motility, and survival. PI3K is comprised of a catalytic subunit, p110, that harbors enzymatic activity and a regulatory subunit, p85, that promotes both p110 stability and inhibition of p85. Moreover, p85 recognizes phosphorylated growth factor receptors via 2 SH2 domains that bring the PI3K dimer to the plasma membrane and in closer proximity to its substrate. Two p85 isoforms, p85α and p85β, have been described. Elevated expression of PIK3R2 (p85β) has been described in breast and colon cancers and is associated with increased tumorigenesis.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human PIK3R2 (aa 426-621)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone failed to immunoprecipitate PIK3R2 from MCF-7 lysates. We therefore do not recommend W17020A for this application.This clone failed to stain PIK3R2 from PFA-fixed and methanol permeabilized MCF-7 cells when tested by ICC. We therefore do not recommend W17020A for this application.Due to pockets of high sequence similarity between the immunizing sequence and PIK3R1, this clone may cross-react with PIK3R1.
RRID: AB_2801126 (BioLegend Cat. No. 667001) AB_2801127 (BioLegend Cat. No. 667002)
Structure: PIK3R2 is a 728 amino acid protein with a predicted molecular weight of 81.5 kD
Distribution: Ubiquitously expressed/cytosolic localization
Function: Cell Signaling
Interaction: p110, FBXL2, PTPN13, XBP1
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Otsu M, et al. 1991. Cell. 65:91. Geering B, et al. 2007. Proc Natl Acad Sci. 104:7809. Cortés I, et al. 2012. Proc Natl Acad Sci. 109:11318. Ito Y, et al. 2014. Proc Natl Acad Sci. 111:16826.
Gene ID: 5296
UniProt: View information about PIK3R2 on UniProt.org
Clone: W17020A
Regulatory Status: RUO
Other Names: Phosphoinositide-3-kinase regulatory subunit 2 P85B
Isotype: Rat IgG2b, κ