Biolegend, 667101, Purified anti-SLP76 Phospho (Ser376) Antibody, 25μg

SLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being tranlocated to the plasma membrane and facilitating the assembly of a multi-subunit complex comprised of Vav1, Nck, ITK, and PLCγ1. SLP76 also plays an essential role in normal T cell development. SLP76 also represents a negative regulatory node in the TCR activation pathway. Following TCR engagement, SLP76 is phosphorylated at Ser376 by the hematopoietic progenitor kinase (HPK1). This modification promotes an interaction between SLP76 and the scaffolding protein 14-3-3ε, resulting in dissociation of the TCR signaling complex and a diminution of TCR signaling.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic human SLP76 peptide containing phosphorylated Ser376
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC on PFA-fixed and methanol permeabilized Jurkat cells treated with or without H2O2 and produced nuclear staining that was not responsive to the treatment. We therefore do not recommend this clone for ICC. †cross-reactivity to Mouse confirmed based on Western blot testing.
RRID: AB_2801128 (BioLegend Cat. No. 667101) AB_2801129 (BioLegend Cat. No. 667102)
Structure: SLP76 is a 533 amino acid protein with a predicted molecular weight of ~60 kDa.
Distribution: Hematopoietic cells/cytosol
Function: Adapter protein/TCR Signaling
Interaction: ITK, LAT, GRB2, PLCγ1, NCK, VAV, ITK, LCK, 14-3-3ε
Cell Type: B cells, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Sauer K, et al. 2001. J Biol Chem. 276:45207. Koretzky GA, et al.. 2006. Nat Rev Immunol. 6:67. Di Bartolo V, et al. 2007. J Exp Med. 204:681. Lasserre, et al. 2011. J Cell Biol. 195:839.
Gene ID: 3937
UniProt: View information about SLP76 Phospho Ser376 on UniProt.org
Clone: A18001A
Regulatory Status: RUO
Other Names: LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein
Isotype: Mouse IgG1, κ