Biolegend, 667451, Purified anti-UPF1 Antibody, 25μg

UPF1 is a crucial RNA helicase and an integral part of the nonsense-mediated mRNA decay (NMD) pathway, a cellular quality control mechanism that ensures the fidelity of gene expression by degrading mRNAs containing premature termination codons. UPF1 contains several key domains that contribute to its function: a N-terminal cysteine-histidine-rich domain (CH domain), a central helicase domain, and a C-terminal domain. The CH domain is involved in regulating the ATPase and helicase activities of UPF1, while the helicase domain facilitates ATP-dependent unwinding of RNA secondary structures. The C-terminal domain interacts with other NMD factors and regulatory proteins. UPF1 facilitates mRNA decay through two primary mechanisms: endonucleolytic cleavage and exonucleolytic decay. SMG6, an endonuclease, directly cleaves the mRNA near the PTC, while SMG5 and SMG7 recruit exonucleases to degrade the RNA from the 5’ and 3’ ends. UPF1's helicase activity is pivotal in remodeling RNA-protein complexes and unwinding secondary structures, thereby allowing exonucleases to efficiently degrade the target mRNA. Beyond its role in NMD, UPF1 is involved in other RNA surveillance and regulatory pathways. It participates in Staufen-mediated mRNA decay (SMD), which targets specific mRNAs bound by the Staufen protein. UPF1 is also implicated in the regulation of histone mRNA decay and telomere maintenance, indicating its versatility in RNA metabolism. Mutations or dysregulation of UPF1 and the NMD pathway have been linked to various human diseases, including cancer, neurodevelopmental disorders, and genetic diseases caused by nonsense mutations.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human UPF1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only This antibody does not cross react with mouse UPF1.
RRID: AB_3675189 (BioLegend Cat. No. 667451) AB_3675189 (BioLegend Cat. No. 667452)
Structure: Contains a N-terminal cysteine-histidine-rich domain (CH domain), a central helicase domain, and a C-terminal domain
Distribution: Ubiquitously expressed in various tissues and cells. Found in the cytoplasm and nucleus
Function: Degrades mRNAs containing premature termination codons, preventing the production of truncated and potentially harmful proteins
Interaction: Participates in post-splicing messenger ribonucleoprotein (mRNP) and exon junction complex (EJC); Interacts with UPF2, UPF3A, UPF3B, SMG5, SMG7, and Staufen1
Molecular Family: Enzymes and Regulators
Antigen References: Kim YK, et al. 2005. Cell. 120:195-208. Chamieh H, et al. 2008. Nat Struct Mol Biol. 15:85-93. Isken O, et al. 2008. Cell. 133:314-27. Hogg JR, et al. 2010. Cell. 143:379-89.
Gene ID: 5976
UniProt: View information about UPF1 on UniProt.org
Clone: W22273D
Regulatory Status: RUO
Other Names: UPF1 RNA Helicase And ATPase, NORF1, PNORF1, Regulator Of Nonsense Transcripts 1, RENT1, Up-Frameshift Suppressor 1 Homolog, Nonsense MRNA Reducing Factor 1, UP Frameshift 1, Delta Helicase, HUpf1, UTF, Smg-2, Q92900
Isotype: Rat IgG2a, κ