Biolegend, 668205, Purified anti-GFP Antibody, 100μg

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea victoria . It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins. This makes GFP a useful tool for monitoring gene expression and protein localization in vivo . Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
100μg
Verified Reactivity: Aequorea victoria, Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed Ting Y, et al. 2017. J Biol Chem. 292(2):585-596. PubMed Nobrega C, et al. 2020. BMC Res Notes. 13:210. PubMed Lin P, et al. 2020. Mol Cell. 78:850. PubMed
RRID: AB_2749908 (BioLegend Cat. No. 668206) AB_2616789 (BioLegend Cat. No. 668205)
Biology Area: Cell Biology
Antigen References: 1. Ishikura H, et al. 2004. Anticancer Res. 24:719. 2. Rizzuto R, et al. 1996. Curr. Biol. 6:183. 3. Chalfie M, et al. 1994. Science 263:802.
Gene ID: NA
UniProt: View information about GFP on UniProt.org
Clone: 1GFP63
Regulatory Status: RUO
Other Names: Green fluorescent protein, GFP
Isotype: Mouse IgG2a