Biolegend, 669602, Purified anti-METTL14 Antibody, 100μg

METTL14 forms a heterodimer with METTL3 called the m6A-methyltransferase complex (MTC) to methylate adenosine residues at the N6 position of eukaryotic mRNAs. MTC-induced methylation takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs. In the MTC complex, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate. Once methylated, the mRNAs are recognized by distinct proteins known as “readers” that will affect their stability, splicing, and/or translation.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial-length recombinant human METTL14 protein between 1-300 a.a.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: While this clone is able to immunoprecipitate METTL14 from whole cell extracts, the resulting fraction was not significantly enriched in METTL14; therefore, this clone may not be suitable for detecting transient or low-abundance complexes.
Product Citations: Liu T, et al. 2022. Cancer Sci. 113:446. PubMed
RRID: AB_2814512 (BioLegend Cat. No. 669601) AB_2814513 (BioLegend Cat. No. 669602)
Structure: METTL14 is a 456 amino acid protein with a molecular weight of 65 kD.
Distribution: Localized to the mitochondria; ubiquitously expressed.
Function: Methylation; mRNA splicing; mRNA translation
Interaction: METTL3
Biology Area: Protein Synthesis
Antigen References: Liu J, et al. 2014. Nat. Chem. Biol. 10.1038/nchembio.1432. Nagase T, et al. 2000. DNA Res. 7:273-81. Yue Y, et al. 2018. Cell Discov. 4:10. Schöller E, et al. 2018. RNA. 10.1261/rna.064063.117. Wang X, et al. 2016. Nature. 10.1038/nature18298.
Gene ID: 57721
UniProt: View information about METTL14 on UniProt.org
Clone: W18219A
Regulatory Status: RUO
Other Names: Methyltransferase Like 14, N6-Adenosine-Methyltransferase Non-Catalytic Subunit, HMETTL14, KIAA1627
Isotype: Rat IgG2a, κ