Biolegend, 669905, PerCP/Cyanine5.5 anti-Cleaved PARP (Asp214) Recombinant Antibody, 25tests

PARP (ADP-Ribose) Polymerase (PARP) is a 113 kD nuclear protein important for mediating the normal cellular response to DNA damage and repair in response to environmental stress. PARP catalyzes the transfer of ADP-ribose units from NAD⁺ to various nuclear proteins, including topoisomerases, histones, and PARP itself, by poly(ADP-ribosyl)ation. Following DNA damage, this protein is cleaved by ICE-like caspases, such as caspase-3 and -7. In humans, this PARP cleavage occurs between Asp214 and Gly215, yielding an 89 kD (from the carboxy-terminal catalytic domain) and a 24 kD fragment (from the amino-terminal DNA binding domain). PARP is an important regulator of cellular differentiation, proliferation, and tumor transformation, and PARP cleavage is considered a classical characteristic of cells undergoing apoptosis. Additionally, this enzyme may be the site of mutation in Fanconi anemia, and may contribute to the pathophysiology of type I diabetes.
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Proprietary synthetic peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC – Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The QA17A17 antibody reacts with the 89 kD (cleaved at Asp214) fragment of human PARP1. It does not react with full-length PARP1. Fixation and permeabilization using the True-Nuclear Buffer Set and Intracellular Staining Permeabilization Wash Buffer (10X) have also been confirmed to work in addition to the True-Phos™ Perm Buffer during in-house testing and development to detect cleaved PARP (Asp214).
RRID: AB_2910493 (BioLegend Cat. No. 669905) AB_2910493 (BioLegend Cat. No. 669906)
Structure: The large fragment of cleaved PARP is an 801 amino acid product with a predicted molecular weight of 89 kD
Distribution: Nuclear
Function: Molecular “nick sensor”; base excision repair; catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture; DNA metabolism; protein modification may enhance or repress transcription
Interaction: Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modified TATA-BP, YY1, Sp1, NF-B, p53 and others
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Satoh M, Lindahl T. 1992. Nature. 356:356. PubMed Kaufmann S, et al. 1993. Cancer Res. 53:3976. PubMed Lazebnik Y, et al. 1994. Nature. 371:347. PubMed D’Amours D, et al. 1999. Biochem J. 342:249. PubMed Chaitanya G, et al. 2010. Cell Commun Signal. 8:31. PubMed
Gene ID: 142
UniProt: View information about Cleaved PARP on UniProt.org
Clone: QA17A17
Regulatory Status: RUO
Other Names: PARP1, Poly (ADP-ribose) polymerase, PPOL, ARTD1, ADPRT, EC 2.4.2, ADP-Ribosyltransferase (NAD+;Poly (ADP-Ribose) Polymerase), Poly (ADP-Ribose) Polymerase Family, Member 1, DNA ADP-Ribosyltransferase PARP1, Poly[ADP-Ribose] Synthase 1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.