Biolegend, 670904, Ultra-LEAF™ Purified anti-GDNF Antibody, 1mg

Glial cell-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor RET receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, which include depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease because it can protect dopaminergic neurons from neurotoxins and promote neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and kidney development. Upregulated expression of GDNF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human GDNF
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding between recombinant human GFRα1 and recombinant human GDNF (Cat. No. 760402). The ND50 range is 0.05 - 0.4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2820112 (BioLegend Cat. No. 670903) AB_2820113 (BioLegend Cat. No. 670904)
Structure: Disulfide-linked homodimer
Distribution: GDNF is a secreted protein that is widely expressed in the developing and adult central nervous system.
Function: GDNF is a neurotrophic factor that promotes the development, differentiation, and the survival of dopaminergic neurons. It also participates in the recovery process after brain surgery.
Interaction: GDNF targets the neuronal cells expressing GDNF family receptors.
Ligand/Receptor: GDNF interacts with GDNF family receptor α-1 (GFRα1) and has a lower affinity with GFRα2, GFRα3, and GFRα4.
Cell Type: Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons, Schwann Cells
Biology Area: Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Growth Factors
Antigen References: Allen SJ, et al. 2013. Pharmacol Ther. 138:155. Hendrich J, et al. 2012. Neuroscience. 219:204. Hidalgo-Figueroa M, et al. 2012. J Neurosci. 32:864. Pascual A, et al. 2011. J Mol Endocrinol. 3:R83-92. Carnicella S, et al. 2009. Pharmacol Ther. 122:9. Sariola H, et al. 2003. J Cell Sci. 116:3855.
Gene ID: 2668
UniProt: View information about GDNF on UniProt.org
Clone: A16099G
Regulatory Status: RUO
Other Names: Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor (ATF), ATF1, ATF2, HSCR3, HFB1-GDNF
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.